Also known as RNA polymerases, the DNA-dependent RNA polymerases are mainly responsible for polymerization of ribonucleotides. The polymerized ribonucleotides are then converted into sequences that are complimentary to that for the template DNA. The main pathways for the enzyme include:
Catalysis of transcription
The primary RNA polymerase is a complex made of five main sub units necessary for transcription processes. The main transcription steps initiated by the enzymes include elongation and termination. DNA-Directed RNA polymerase catalyzes the reactions involving RNA (n) and nucleoside triphosphateforming RNA (n+1) and diphosphate.
It catalyzes the DNA template chain de novo whereby it utilizes the DNA template. The catalysis of the main DNA template-directed extension of the 3’ end of the RNA strand one nucleotide, in sequence. Any deformations or defects on this enzyme will affect major molecular and biological activities in the body and can be detected by an ELISA test kit specifically made for the rpol enzyme/ recombinant protein.
Purine metabolism
Purine metabolism is a very complicated process requiring the DNA-directed RNA polymerase for synthesis of RNA and eventually DNA. Purine anabolism and catabolism are both very important for transcription processes to take place.
Any deformations as a result of mutations or deficiency of the enzyme results in in levels of purine metabolic pathway antibodies. There also could be absence of antibodies in case of deficiencies in the enzyme.
Pyrimidine metabolism
The DNA directed RNA polymerase enzyme facilitates the transcription process leading to the sequential addition of nucleotides to the RNA’s 3’ Strand. The nucleotides are either purines or pyrimidines and deficiency of the enzymes will affect the metabolism and addition of the pyrimidines to the strands.
Other roles of DNA directed RNA polymerase
This recombinant protein tested using ELISA kits catalyzed the molecular process involving transcription of mitochondrial DNA to RNA using four ribonucleoside triphosphates as the main substrate. The main molecular function carries out by the enzymes include DNA-directed polymerase activity, DNA binding and protein binding. Biological processes also require the enzyme for functions such as gene expression, initiation and transcription from the mitochondrial promoter.
As a result, absence of the enzymes does inhibit many important processes in the cells. Eukaryotes particularly depend widely on the enzyme for biogenesis and organelle organizations. Metal ion binding for magnesium and zinc are also dependent on the enzyme.
Viral infections and diseases such as HIV are detected through ELISA testing where the viral elements are detected through the complex yet specific antigen-antibody interactions. This recombinant protein is therefore important in immune activity and antibodies in immune pathways.
In conclusion, this recombinant protein is important for researches done in laboratories to ascertain the pathways with issues or when finding problems. Defects and mutations to the enzyme will affect many biological and molecular pathways in organisms and though such incidences are rare, when they occur, they have lethal effects and no life can be sustained.
You should note that small volumes of the lab recombinant protein may be entrapped in the vial seal during shipment. Therefore, you may have to centrifuge the vial on a table top centrifuge to dislodged trapped proteins.